Room 401 was a hidden camera/reality television series on MTV, executive produced by Ashton Kutcher and Jason Goldberg. It was named after the room Harry Houdini died in at Detroit's Grace Hospital in 1926. The show also used some of his famous acts.

Room 401 took unaware victims into the center of pranks that played like mini horror movies. From the reanimation of the dead, to chain-saw mishaps, each episode contains four "scares" or pranks. The show was hosted by Jared Padalecki from the CW's Supernatural.